(Sobre)viver na rua: narrativas das pessoas em situação de rua sobre a rede de apoio / (About) living on the street: narratives of homeless people about the support network / (Sobre)vivir en la calle: narrativas de personas en situación de calle sobre la red de apoyo

A população em situação de rua (PSR), em seu cotidiano, se relaciona com diferentes pessoas, grupos e/ou coletivos ligados à execução das políticas públicas, às organizações não governamentais, familiares ou a membros da sociedade civil. Pensar nessas dinâmicas de trabalho, cooperação e auxílio remete a pensar sobre uma rede de apoio que constrói estratégias com essa população. Tendo presente essas problematizações, este estudo teve como objetivo analisar as narrativas das pessoas em situação de rua sobre como é produzida sua rede de apoio. Para tanto, foi realizado um estudo qualitativo, de orientação etnográfica, sendo utilizada a observação participante, registros em diário de campo e entrevistas narrativas. Participaram seis pessoas em situação de rua que recebem alimentação ofertada por projetos sociais em uma cidade do interior do Rio Grande do Sul. Os dados produzidos foram analisados a partir da Análise Temática. As análises expressam as especificidades das narrativas das trajetórias de vida associadas à chegada às ruas e à composição de uma rede de apoio na rua. Ao conhecer como se produz e opera essa rede de apoio, a partir das narrativas das pessoas em situação de rua, problematiza-se a complexidade dessa engrenagem e o desafio de produzir ações integradas entre as diferentes instâncias da rede. Nisso, destaca-se a potencialidade de práticas que levem conta à escuta, ao diálogo e à articulação na operacionalização de políticas públicas atentas às necessidades dessa população.(AU)

The street population, in their daily lives, relates to different people, groups and/or collectives linked to the execution of public policies, to non-governmental organizations, family members, or to members of civil society. Thinking about these dynamics of work, cooperation, and assistance leads to thinking about a support network that builds strategies with this population. Having these problematizations in mind, this study aims to analyze the narratives of homeless people about how their support network is produced. To this end, a qualitative study was carried out, with ethnographic orientation, using participant observation, records in a field diary, and narrative interviews. Participated in the research six homeless people who receive food offered by social projects in a municipality in the interior of Rio Grande do Sul. The data produced were analyzed using the Thematic Analysis. The analyzes express the specifities of the narratives of life trajectories associated with the arrival on the streets and the composition of a support network on the street. By knowing how the support network is produced and operated, the complexity of this gear and the challenge of producing integrated actions between the different instances of the network are problematized. Thus, it highlights the potential of practices that consider listening, dialogue, and articulation in the operationalization of public policies that are attentive to the needs of this population.(AU)

Las personas en situación de calle en su cotidiano se relacionan con distintas personas, grupos y/o colectivos, que están vinculados a la ejecución de políticas públicas, organizaciones no gubernamentales, familiares o miembros de la sociedad civil. Pensar en estas dinámicas de trabajo, cooperación y ayuda nos lleva a una red de apoyo que construye estrategias con estas personas. Teniendo en cuenta esta problemática, este estudio tiene como objetivo analizar las narrativas de las personas en situación de calle acerca de cómo se produce su red de apoyo. Con este fin, se realizó un estudio cualitativo, etnográfico, utilizando observación participante, registros de diario de campo y entrevistas narrativas. Este estudio incluyó a seis personas en situación de calle que reciben alimentos ofrecidos por proyectos sociales en una ciudad del interior de Rio Grande do Sul (Brasil). Se utilizó el Análisis Temático. Los análisis expresan las especificidades de las narrativas de las trayectorias de vida asociadas con la llegada a las calles y la composición de una red de apoyo en la calle. Al saber cómo se produce y opera la red de apoyo, a partir de las narrativas de las personas en la calle, se problematizan la complejidad de este equipo y el desafío de producir acciones integradas entre las diferentes instancias de la red. Destaca el potencial de las prácticas que tienen en cuenta la escucha, el diálogo y la articulación en la implementación de políticas públicas que estén atentas a las necesidades de esta población.(AU)

Ayuno intermitente como opción terapéutica para pacientes con sobrepeso y obesidad / Intermittent fasting as a therapeutic option for overweight and obese patients

Los regímenes basados en la restricción intermitente de la ingesta de alimentos, como el ayuno intermitente, pueden parecer novedosos pero constituyen en realidad una práctica milenaria. Muchas veces en nuestras consultas como médicos de familia los pacientes con problemas de sobrepeso u obesidad nos preguntan sobre estas prácticas y sus efectos en la salud. A partir de la viñeta clínica de uno de esos pacientes, revisamos la evidencia disponible sobre el tema y encontramos que la restricción intermitente puede ser una intervención eficaz para la disminución de peso, aunque sin diferencias significativas con respecto a la restricción continua. Para otros desenlaces analizados, como el riesgo cardiovascular, la evidencia no es tan clara. Si bien la restricción alimentaria intermitente puede ser una opción útil en para los pacientes que desean disminuir su peso, se necesitan más estudios para determinar la variante más adecuada, su duración óptima, la mejor frecuencia semanal y sus beneficios a largo plazo. (AU)

Intermittent dietary restriction, like intermittent fasting, may seem like a novel diet, but it's actually an age-old practice. Many times in our practice as family physicians, patients with overweight or obesity problems ask us about this practice and its effects on health. From the clinical vignette of one of our patients, we reviewed the available evidence on the subject and found that intermittent dietary restriction could be an effective intervention for weight loss, but without significant differences with respect to continuous caloric restriction. For other outcomes analyzed, such as cardiovascular risk, the evidence is not as clear. Al though intermittent dietary restriction may be a useful option in our patients, more studies are needed to determine which variant is the most appropriate, its optimal duration, weekly frequency, and long-term benefits. (AU)

Efectos de la actividad física sobre el consumo y selección de alimento en ratas que fueron expuestas a restricción alimentaria durante la gestación / Effects of physical activity on the consumption and selection of food in rats that were exposed to food restriction during pregnancy

Las alteraciones durante la vida prenatal tienen diversos efectos en los organismos. La restricción alimentaria materna ocasiona modificaciones en la conducta alimentaria como hiperfagia y su exacerbación ante la exposición a una dieta hiperlipídica. La evidencia experimental indica que aun cuando existe una preferencia por los alimentos altos en grasa, cuando las ratas realizan actividad física, esta preferencia disminuye o se elimina. Objetivo: evaluar el efecto de la restricción alimentaria materna sobre el consumo de una dieta suplementada con nuez pecana y cómo influye la actividad física. El experimento incluyó 22 ratas, 11 del grupo experimental y 11 del grupo control. De los cuales 6 realizaron actividad y 5 permanecieron sedentarias en cada grupo (machos y hembras). El experimento duró 114 días, de los cuales 42 días tuvieron disponible la rueda de actividad. Los resultados mostraron que la restricción alimentaria materna no modificó el comportamiento alimentario, sin embargo, cuando incrementaron la actividad por la disponibilidad de la rueda de actividad, los sujetos experimentales aumentaron su consumo de nuez pecana. Los resultados se consideran contradictorios con respecto a la literatura, ya que muestran ausencia de hiperfagia e incremento en el consumo a la par del incremento en actividad física.

Alterations during prenatal life have various effects on organisms. Maternal food restriction causes changes in feeding behavior such as hyperphagia and its exacerbation when exposed to a hyperlipidic diet. Experimental evidence indicates that even when there is a preference for high-fat foods, when rats do physical activity, this preference decreases or is eliminated. Objective: to evaluate the effect of maternal dietary restriction on the consumption of a diet supplemented with pecan nuts and how physical activity influences this relationship. The experiment included 22 rats, 11 experimental and 11 controls. Of these, 6 performed physical activity and 5 remained sedentary in each group (males and females). The experiment lasted 114 days; the activity wheel was available on 42 days. The results showed that maternal food restriction did not modify eating behavior, however, when rats increased physical activity, experimental subjects increased their consumption of pecan nuts. The results are contradictory with respect to the literature, as they show an absence of hyperphagia and an increase in consumption along with an increase in physical activity.

Is the colon mucosa affected by ten days of gastric restriction in an animal model?

Abstract Purpose To identify whether the colon mucosa is affected by ten days of gastric restriction in an animal model. Methods An experimental model of gastric restriction was devised using rats. The animals were submitted to surgical gastrostomy, and a cylindrical loofah was inserted into the stomach. We studied 30 adult male Wistar rats divided into three groups: the stomach restriction group (R10); the sham group (S10), which underwent the same procedure except for the loofah insertion; and the control group (C10). The expression of neutral and acid mucins was evaluated using histochemical techniques. Goblet cells and protein content were compared between groups using generalized estimation equations (GEEs). Bonferroni's multiple comparison was applied to identify differences between the groups. All tests considered a 5% significance level. Results There was an increased expression of neutral mucins, acid mucins and goblet cells in the R10 group. Collagen was also enhanced in the R10 group. Conclusion The colon mucosa is affected by ten days of gastric restriction in an animal model, increasing neutral mucins, acid mucins and collagen content with trophic maintenance.

Efeito do valor reforçador do alimento em diferentes tempos de privação alimentar / Effect of food reinforcement value at different deprivation periods

Avaliar o valor reforçador do alimento sob diferentes tempos de privação; até 4 horas (grupo tempo 1 - GT1) e mais de 4 horas (grupo tempo 2 - GT2), em mulheres e homens eutróficos e com excesso de peso, classificados pelo índice de massa corporal (IMC). Foram ouvidos 258 adultos, sendo 126 homens e 132 mulheres (18 - 50 anos), foram divididos em GT1 ou GT2. Após classificarem a fome pela escala analógica visual 100 mm, realizaram um jogo desenvolvido para este estudo (software Food or Fun 1.0), onde deveriam trabalhar para ganhar pontos em alimento ou lazer. Na amostra geral o relato de fome diferiu estatisticamente (p<0,001) entre GT1 (42,4 ± 25,9) e GT2 (60,2 ± 22,4) assim como a pontuação em alimentos (GT1=26,7 ± 23,5; GT2=35,2 ± 23,4; p<0,001), demonstrando uma tendência diretamente proporcional ao tempo de privação em ambos os sexos. Uma correlação negativa significativa foi encontrada entre IMC e pontos em alimentos para a amostra geral (r=-0,16; p=0,013) e do sexo feminino (r=-0,18; p=0,040). O valor reforçador do alimento aumentou concomitantemente com o tempo de privação e IMC apresentou uma correlação inversa à pontuação em alimentos.

Current paper evaluates the reinforcing value of food at different deprivation periods: up to 4 h (Group Time 1 - GT1) and more than 4 h (Group Time 2 - GT2), in eutrophic females and males, weight excess, classified by Body Mass Index (BMI). Divided into GT1 and GT 2, 258 adults, 126 males and 132 females, age bracket 18 -50 years, were interviewed. After the classification of fasting by the 100m visual analogical scale, a game was developed for current study (software Food or Fun 1.0), in which they had to work for scores in food or fun. Within the general sample, there was a statistical difference in fasting (p<0.001) between GT1 (42.4 ± 25.9) and GT2 (60.2 ± 22.4) and in feeding scores (GT1=26.7 ± 23.5; GT2=35.2 ± 23.4; p<0.001), with a proportional trend to deprivation time in both genders. A significant negative co-relationship was detected between BMI and feeding scores for general sample (r=-0.16; p=0.013) and females (r=-0.18; p=0.040). Reinforcing value of feeding increased concomitantly with privation period and BMI had an inverse co-relationship to feeding scores.

Food deprivation does not affect growth performance of juvenile tambacu / Privação alimentar não afeta o crescimento de juvenis de tambacu

The objective of this study was to evaluate the efficiency of food deprivation on the growth performance of the commercially important hybrid fish tambacu (Piaractus mesopotamicus male × Colossoma macropomum female). For this, 171 juveniles of average length 8 cm and 11.4 g body mass were distributed in nine tanks, and allocated to three treatments: the fish were fed every day (control), fed for six days followed by one day of food deprivation (6F/1D) and fed for five days followed by two days of food deprivation (5F/2D). After 77 days, all animals achieved similar growth rates. Only animals submitted to two days of food deprivation presented hyperphagia. There was a reduction in total feed consumption of 3.8% and 10.8% for the 6F/1D and 5F/2D treatments, respectively. Our results suggest that juvenile tambacu exhibit total compensatory growth when submitted to food deprivation, and that a two-day deprivation could significantly reduce production costs for tambacu farmers.

O objetivo do estudo foi avaliar a eficiência da privação alimentar sobre o desempenho zootécnico de tambacu (Piaractus mesopotamicus x Colossoma macropomum). Para isso, 171 animais (massa: 11,4 ± 1.5 g; comprimento total: 8.0 ± 0.4 cm) foram alocados em nove tanques (19 peixes/tanque; n=3). Os peixes foram alimentados todos os dias (controle), alimentados durante seis dias seguidos de um dia de privação alimentar (6F/1D) e durante cinco dias seguidos de dois dias de privação alimentar (5F/2D). Após 77 dias, todos os animais alcançaram índices de crescimento similares. Somente os animais submetidos a dois dias de privação apresentaram hiperfagia. Houve redução do consumo total de ração em 3,8 e 10,8% para os tratamentos 6F/1D e 5F/2D, respectivamente. Os nossos resultados demonstram que os juvenis de tambacu exibiram crescimento compensatório total quando submetidos a privação alimentar, e a estratégia de 2 dias de privação alimentar pode reduzir significativamente os custos de produção de tambacu.

Interleukin-12 as a biomarker of the beneficial effects of food restriction in mice receiving high fat diet or high carbohydrate diet

The impact of food restriction (FR) during 56 days on serum levels of cytokines in mice fed a high-fat diet (HFD) or high-carbohydrate diet (HCD) were evaluated. The amount of food was reduced 50% for HFD-FR and HCD-FR groups compared to mice receiving free access to HFD (HFD group) or HCD (HCD group). We quantified the serum levels of basic fibroblast growth factor, granulocyte-macrophage colony-stimulating factor, inducible protein 10, interferon γ, interleukin 1α (IL-1α), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant, macrophage inflammatory protein-1α, monocyte chemotactic protein 1, monokine induced by IFN-γ, and tumor necrosis factor α. Only IL-12 levels were lower (P<0.05), for both HFD-FR (HFD-FR vs HFD) and HCD-FR (HCD-FR vs HCD). Therefore, IL-12 levels could be considered a biological marker of the beneficial effects of FR.

Rebound Feeding in the Wake of Short-Term Suspension of Food Intake Differs in the Presence of Estrous Cycle Peak versus Nadir Levels of Estradiol

BACKGROUND: Short-term interruption of feeding is ordinary in modern life but negatively impacts appetite control and body weight. Estradiol (E) imposes long-term inhibitory tonus on food consumption; however, E influence on energy repletion secondary to food deprivation (FD) is unclear. This study investigated the hypothesis that E signal strength regulates hyperphagic responses to FD of varying duration. METHODS: Ovariectomized female rats were implanted with E-containing silastic capsules (30 [E-30] or 300 µg [E-300]/mL) to replicate plasma concentrations at cycle nadir versus peak levels. RESULTS: Data show that food intake was increased equally in E-30 and E-300 rats after 12 hours of food deprivation (FD-12); yet, FD of 18 hours (FD-18) amplified refeeding by E-300 versus E-30. Caudal fourth ventricular administration of the 5′-monophosphate-activated protein kinase (AMPK) inhibitor compound C (Cc) did not modify FD-induced hyperphagia in E-30 (regardless of FD interval) or E-300 animals exposed to FD-12, but diminished refeeding after FD-18 in E-300 rats. Cc-reversible hyperglycemia occurred in refed FD-18 groups. Serum insulin was resistant to FD-12 plus refeeding, but was elevated by AMPK-dependent mechanisms in refed E-300 FD-18 rats; equivalent Cc-insensitive decrements in circulating leptin occurred in all FD groups. CONCLUSION: Current results show that estrous cycle peak, but not baseline, E levels engage hindbrain AMPK signaling to intensify hyperphagia in response to prolongation of FD. Observations of hindbrain AMPK-dependent hyperglycemia, alongside elevated insulin secretion, in refed rats exposed to FD-18 implicate this sensor in insulin resistance mechanisms of glucose partitioning in response to this metabolic imbalance.

Insegurança alimentar em povos de terreiros / Food insecurity in people of terreiros

INTRODUÇÃO: O conceito de segurança alimentar é compreendido como a realização do direito de todos ao acesso regular e permanente a alimentos de qualidade em quantidade suficiente, sem afetar outras necessidades essenciais. OBJETIVO: Estimar a prevalência de insegurança alimentar intrafamiliar em povos de terreiros e verificar a associação com indicadores socioeconômicos. MÉTODOS: Estudo transversal que investigou a situação de insegurança alimentar em povos de terreiros de Teresina, Piauí, cuja amostra foi constituída por 137 casas ativas no período de agosto de 2011 a julho de 2012. Foram instrumentos de coleta de dados a Escala Brasileira de Insegurança Alimentar e um questionário sociodemográfico aplicados ao pai/mãe de santo, após consentirem formalmente sua participação na pesquisa. Para análise das variáveis e da insegurança alimentar, foi utilizado o teste χ2. RESULTADOS: A insegurança alimentar esteve presente em 79,6% dos domicílios, sendo 29,9% de insegurança alimentar leve, 33,6% na forma moderada e 16,1% na grave. Nos domicílios com menores de 18 anos, a prevalência de insegurança alimentar grave foi maior (22,7%) se comparada aos domicílios que possuíam moradores com 18 anos ou mais (14,7%). As variáveis independentes como rendimento mensal e tipo de moradia apresentaram associação inversamente significante à insegurança alimentar. CONCLUSÃO: Os resultados expressam a gravidade da situação de insegurança alimentar em povos de terreiro em Teresina, indicam a violação do direito humano à alimentação adequada vivenciada por esse grupo e reforçam a necessidade do poder público agir mais efetivamente com ações de proteção social junto a esses povos.

INTRODUCTION: The concept of food security is understood as the realization of everyone’s right to regular and permanent access to quality food in sufficient quantity, without affecting other essential needs. OBJECTIVE: To estimate the prevalence of intrafamily food insecurity in people of terreiros and verify the association with socioeconomic indicators. METHODS: Cross-sectional study that investigated the food insecurity of people in terrains in Teresina, Piauí, Brazil. The sample consisted of 137 active houses from August 2011 to July 2012. The Brazilian Food Insecurity Scale and the sociodemographic questionnaire were the instruments of data collection applied to pai/mãe de santo, after formally consent to their participation in the research. To analyze variables and food insecurity, the χ2 test was used. RESULTS: Food insecurity was present in 79.6% of households, with 29.9% in mild food insecurity, 33.6% in moderate and 16.1% in severe. In households with children under 18 years of age, the prevalence of severe food insecurity was higher (22.7%) than in households with residents who were 18 years old or older (14.7%). Independent variables such as monthly income and housing type showed significant association with food insecurity. CONCLUSION: The results express the severity of food insecurity of people in the terrains of Teresina, Piauí, Brazil, indicating a violation of the human right to adequate food experience in this group and reinforcing the necessity for public authorities to act more effectively with social protection actions for these people.

Protective effect of melatonin against inequality-induced damages on testicular tissue and sperm parameters

The goals of the study are evaluation of the effects of food deprivation and isolation situation as a social stress on fertility; and in the following, investigation of the improving effect of melatonin as an antioxidant component. In this experimental study, We investigated histopathological and serological effects of melatonin and social stress [food deprivation and isolation] on different features of sperm and testicular tissue among 42 male rats in 7 groups including control, sham, melatonin received [M], food deprivation [FD], Food deprivation and melatonin treatment [FDM], Food deprivation and isolation situation [FDi], and Food deprivation and melatonin treatment and isolation situation [FDMi] groups. Epididymal sperms of all rats were also counted. Histopathological evaluation of the testes was done under a light microscopy to determine the number of spermiogenic cells. Serological evaluation of testosterone, corticosterone, and melatonin was performed, as well. For statistical analysis, one-way ANOVA and Tukey's post hoc test were used, and the value of p

How does protein malnutrition or food deprivation interfere with the growth of the epiphyseal plate in animals? / Cómo interfieren la desnutrición protéica o la privación alimentaria en el crecimiento de la placa epifisaria de animales?

The aim of this study was to conduct a literature review of impacts of protein malnutrition and/or early food deprivation on the formation of the growth plate in young animals. A search was performed using the databases PubMed/MEDLINE, LILACS, SciELO and the Cochrane Library, without language restrictions or any limit as to year. Initially, 150 articles were identified, however, after application of the inclusion and exclusion criteria only five remained. In three studies the nutritional stress was due to protein malnutrition, another due to fasting and the fifth was due to food restriction. All the studies had some methodological omissions. The studies included in this review-demonstrated corroboration in the results obtained, regardless of whether the animals had been subjected to protein malnutrition, fasting or food restriction. The findings uncovered were reduction in height of the epiphyseal plate, in the number of proliferative and hypertrophic chondrocytes, in the bone growth rate and in the longitudinal length of the bone in animals subjected to nutritional stress. In this systematic review, it was possible to observe the susceptibility of the epiphyseal plate in the first place, and secondarily, of the long bones to the effects of nutritional stress by means of protein malnutrition or food deprivation applied in young animals.

El objetivo fue realizar una revisión de la literatura sobre el impacto de la desnutrición proteica y/o privación de alimentos en la temprana formación de la placa de crecimiento en animales jóvenes. La búsqueda fue realizada en las bases de datos PubMed/MEDLINE, LILACS, SciELO y Biblioteca Cochrane sin restricción de lengua o límite de año. Fueron identificados inicialmente ciento cincuenta artículos que, posterior a la aplicación de los criterios de inclusión y exclusión, solo quedaron cinco. En tres estudios el estrés nutricional fue causado por la desnutrición proteica, otro al ayuno y el tercero fue por restricción alimentaria. Todos los estudios presentaban algunas omisiones metodológicas. Las investigaciones incluidas en esta revisión demostraron concordancia en sus resultados obtenidos, independientemente que los animales hayan sido sometidos a desnutrición proteica, ayuno o restricción alimentaria. Los datos obtenidos mostraron que los animales sometidos al estrés nutricional presentaron una reducción en la altura de la placa epifisaria, en el número de condrocitos proliferativos e hipertróficos, en la tasa de crecimiento óseo y en la longitud ósea. En esta revisión sistemática fue posible observar que los efectos del estrés nutricional, a través, de desnutrición proteica o de la privación alimentaria aplicada en animales jóvenes presentan, en primer lugar, una susceptibilidad de la placa epifisaria y de los huesos largos de manera secundaria.

Effect of Food Deprivation on a Delayed Nonmatch-to-place T-maze Task

Food deprivation can affect performance on difficult cognitive task, such as the delayed nonmatch-to-place T-maze task (DNMT). The importance of food deprivation on maintaining high motivation for DNMT task has been emphasized, but not many studies have investigated the optimal conditions for depriving rodents to maximize performance. Establishing appropriate conditions for food deprivation is necessary to maintain DNMT task motivation. We applied different conditions of food deprivation (1-h food restriction vs. 1.5-g food restriction; single caging vs. group caging) and measured body weight and the number of correct choices that 8-week-old C57BL/6J mice made during the DNMT task. The 1.5-g food restriction group maintained 76.0+/-0.6% of their initial body weight, but the final body weight of the 1-h food restriction condition group was reduced to 62.2+/-0.8% of their initial body weight. These results propose that 1.5-g food restriction condition is effective condition for maintaining both body weight and motivation to complete the DNMT task.

cAMP/PKA Agonist Restores the Fasting-Induced Down-Regulation of nNOS Expression in the Paraventricular Nucleus

Gene expression of neuronal nitric oxide synthase (nNOS) changes in the hypothalamic paraventricular nucleus (PVN) depending on feeding conditions, which is decreased during food deprivation and restored by refeeding, and phosphorylated cAMP response element binding protein (pCREB) was suggested to play a role in its regulation. This study was conducted to examine if the fasting-induced down-regulation of the PVN-nNOS expression is restored by activation of cAMP-dependent protein kinase A (cAMP/PKA) pathway. Freely moving rats received intracerebroventricular (icv) injection of cAMP/PKA activator Sp-cAMP (40 nmol) or vehicle (sterilized saline) following 48 h of food deprivation. One hour after drug injections, rats were transcardially perfused with 4% paraformaldehyde, and the PVN tissues were processed for nNOS or pCREB immunohistochemistry. Sp-cAMP significantly increased not only nNOS but also pCREB immunoreactivities in the PVN of food deprived rats. Fasting-induced down-regulation of the PVN-nNOS was restored by 1 h after the icv Sp-cAMP. Results suggest that cAMP/PKA pathway may mediate the regulation of the PVN-nNOS expression depending on different feeding conditions.

Influence of food restriction on lipid profile and spontaneous glucose levels in male rats subjected to paradoxical sleep deprivation

OBJECTIVES: The purpose of this study was to determine the paired consequences of food restriction and paradoxical sleep deprivation on lipid profile and spontaneous glucose levels in male rats. METHOD: Food restriction began at weaning, with 6 g of food being provided per day, which was subsequently increased by 1 g per week until reaching 15 g per day by the eighth week. At adulthood, both rats subjected to food restriction and those fed ad libitum were exposed to paradoxical sleep deprivation for 96 h or were maintained in their home-cage groups. RESULTS: Animals subjected to food restriction exhibited a significant increase in high-density lipoprotein levels compared to animals that were given free access to food. After the paradoxical sleep deprivation period, the foodrestricted animals demonstrated reduced concentrations of high-density lipoprotein relative to their respective controls, although the values for the food-restricted animals after sleep deprivation were still higher than those for the ad libitum group. The concentration of low-density lipoproteins was significantly increased in sleep-deprived animals fed the ad libitum diet. The levels of triglycerides, very low-density lipoproteins, and glucose in foodrestricted animals were each decreased compared to both ad libitum groups. CONCLUSION: These results may help to illustrate the mechanisms underlying the relationship between sleep curtailment and metabolism and may suggest that, regardless of sleep deprivation, dietary restriction can minimize alterations in parameters related to cardiovascular risk.

[Study of the therapeutic effect of melatonin after food deprivation in apoptosis and oxidative stress in rat testis]

The goals of the study are evaluation the effect[s] of food deprivation as a social stress on testis structure. We also investigated the effects of melatonin treatment as an antioxidant component and inequality on the effect[s] of food deprivation. We investigated the improving effects of melatonin and social stress [food deprivation] on 42 male rats in 7 groups including control, sham, melatonin received [M], food deprivation [1/3 of control daily food] plus observation [FD], FD + melatonin [FDM], isolated FD [FDi], and FDi + melatonin [FDMi] groups. After 14 days, rats' testes were studied using immuno histochemistry and TUNEL assays to determine the number of apoptotic cells. Biochemical evaluation was taken on malodialdehide [MDA] and glutathione [GSH]. ANOVA and Tukey's tests were done to analyse the data. P<0.05 was considered statistically significant. The results of sham group was declined for similarity to results of control group. In FD group, MDA was increased significantly [P<0.01], GSH was decreased and the number of apoptotic cells was increased, significantly [P<0.01]. In FDi group, there was no effect on the ratio of oxidative stress compared to the control group. Melatonin treatment could decrease apoptotic cells [P<0.05] and MDA concentration [P<0.05] in the FD group. Food deprivation can induce oxidative stress which is associated with increasment of apoptotic cells in testis. Isolation can compensate these effects. These results refer to inequality. Since melatonin is recognized for its anti-oxidative and improving effects, we have shown involvement of oxidative stress mechanisms on the stress of food deprivation with inequality

The effect of melanocortin (Mc3 and Mc4) antagonists on serotonin-induced food and water intake of broiler cockerels

The current study was designed to examine the effects of intracerebroventricular injections of SHU9119 [a nonselective melanocortin receptor (McR) antagonist] and MCL0020 (a selective McR antagonist) on the serotonin-induced eating and drinking responses of broiler cockerels deprived of food for 24 h (FD24). For Experiment 1, the chickens were intracerebroventricularly injected with 2.5, 5, and 10 microg serotonin. In Experiment 2, the chickens received 2 nmol SHU9119 before being injected with 10 microg serotonin. For Experiment 3, the chickens were given 10 microg serotonin after receiving 2 nmol MCL0020, and the level of food and water intake was determined 3 h post-injection. Results of this study showed that serotonin decreased food intake but increased water intake among the FD24 broiler cockerels and that these effects occurred in a dose-dependent manner. The inhibitory effect of serotonin on food intake was significantly attenuated by pretreatment with SHU9119 and MCL0020. However, the stimulatory effect of serotonin on water intake was not altered by this pretreatment. These results suggest that serotonin hypophagia and hyperdipsia were mediated by different mechanisms in the central nervous system, and that serotonin required downstream activation of McRs to promote hypophagia but not hyperdipsia in the FD24 chickens.

Maternal Food Restriction During Lactation Reduces Mandible Growth of the Female Offspring in Adulthood: Experimental and Morphometric Analysis / Restricción Alimentaria Materna Durante la Lactancia Reduce el Crecimiento de la Mandíbula de las Crías Hembras en la Edad Adulta: Análisis Experimental y Morfométrico

The aim of this study was to evaluate the effects of maternal protein and energy restriction during lactation on mandible dimensions of pups (female) at aging. At parturition, Wistar rat dams were randomly assigned to the following groups: 1) control group (C) - free access to a standard laboratory diet containing 23 percent protein, 2) protein-energy restricted group (PER) - free access to an isoenergetic, protein-restricted diet containing 8 percent protein, and 3) energy-restricted group (ER) ­ fed with restricted amounts of a standard laboratory diet. At weaning (d21), all pups were separated of dams and received free access to a standard laboratory diet containing 23 percent protein until 90 days (d90), when the rats were anesthetized and sacrificed. The dimensions of excised pup mandible were measured directly using pre-established 3 anatomical points. Morphometric analysis showed that most of the measurements in the ER and PER groups were significantly lower than in the control group, with the greatest reductions occurring in the PER group. These results show that protein and energy restriction during lactation have an important influence on pup mandible development and continue through the aging process.

El objetivo de este estudio fue evaluar los efectos de la restricción de la proteína materna y de la energía durante la lactancia sobre las dimensiones de la mandíbula de crías (hembras) durante la vida. En el parto, las crías de ratas Wistar fueron agrupadas aleatoriamente en los siguientes grupos: 1) control (C) - con acceso libre a una dieta estándar del laboratorio, que contenía 23 por ciento de proteínas; 2) con restriccion de proteínas y energía (PER) - acceso libre a una dieta isoenergética, con restricción de proteínas, conteniendo un 8 por ciento de éstas y 3) grupo con restricción de energía, alimentado con restricción en la cantidad de alimento de la dieta estándar del laboratorio (ER). Al destete, todas las crías fueron separadas y recibieron las dietas establecidas hasta los 90 días (d90), luego fueron anestesiadas y sacrificadas. Las mediciones de las mandíbulas de las crías de rata fueron obtenidas por 3 puntos anatómicos preestablecidos. El análisis morfométrico de la mandíbula demostró que la mayoría de las mediciones en los grupos ER y PER eran significativamente menores que las del grupo control, con mayores reducciones en el grupo PER. Estos resultados muestran que las restricciones de energía y proteínas durante la lactancia tienen una influencia importante en el desarrollo de la mandíbula de las crías, las que se mantuvieron con el paso del tiempo.

Effects of Food Deprivation on the Development of Coleomegilla maculata (De Geer) (Coleoptera: Coccinellidae)

The lady beetle Coleomegilla maculata (De Geer) is a natural enemy of several insect pests and feeds on pollen and nectar to survive periods when prey is scarce. The effect of the feeding interval on the development, survival, fecundity, and longevity of C. maculata was determined. Newly hatched larvae of C. maculata were reared individually and fed with eggs of the Mediterranean flour moth Anagasta kuehniella (Zeller) at intervals of one, two, and three days under controlled conditions (23 ± 1ºC; 60 ± 10 percent RH; 12 h phtophase). The duration of larval instars and the total larval stage was prolonged as the feeding interval increased. The larval period lasted on average 9.2 ± 0.19 days when the larvae were fed daily with prey, and 14.6 ± 0.48 days when food was offered at three-day intervals. There was an inverse relationship between food intervals, survival, and weight of larvae and adults of the coccinellid. Survival rate of larvae fed daily was 76.8 percent, while the rate was 50.0 percent and 23.4 percent for larvae fed every two and three days, respectively. Coleomegilla maculata showed fecundity of 781.1 ± 149.02, 563.4 ± 80.81 and 109.0 ± 103.0 eggs when fed daily and at intervals of two and three days, respectively.

Padrões alimentares, participação em programas sociais e demais fatores associados à insegurança alimentar e nutricional em adolescentes de escolas públicas no município de Piracicaba, São Paulo / Food patterns, participation in social programs and other factors related to food insecurity among adolescents enrolled in public schools of Piracicaba

Objetivo. Verificar a existência de associações entre a insegurança alimentar e nutricional e padrões alimentares, a participação em programas sociais, dentre outros fatores em adolescentes de escolas públicas de Piracicaba (SP). Métodos. Este estudo foi conduzido em amostra probabilística de 488 escolares da 5ª série do ensino fundamental da rede pública de Piracicaba. Foram coletadas informações demográficas, antropométricas, bioquímicas e de pressão arterial, sobre a adesão à alimentação escolar, e de consumo alimentar a partir de Questionário de Frequência Alimentar Simplificado para Adolescentes (QFASA). Também foram obtidas informações sobre as situações de insegurança alimentar e nutricional (IAN), participação em programas de transferência de renda e informações socioeconômicas. Foram realizadas análises descritivas, testes de Qui-quadrado de Pearson, t de Student e U de Mann Whitney. Padrões alimentares foram definidos a partir de Análise de Componentes Principais. A análise de regressão logística avaliou a influência de múltiplas variáveis na presença de IAN (variável dependente dicotômica). O estudo foi aprovado pelo Comitê de Ética da Faculdade de Saúde Pública. Resultados. De 308 escolares da amostra final, 54,5 por cento eram meninas. Mais de 40 por cento dos lares de escolares entrevistados apresentaram algum nível de IAN. Observaram-se elevadas proporções de adesão a alimentação escolar (70 por cento) e de excesso de peso (39 por cento). O principal padrão alimentar observado contou com maior participação de doces, massas, carnes, salgados, pães, frutas e hortaliças.

[Effects of short-term starvation on hematological parameters in cultured juvenile beluga]

This study examined the effect of different starvation periods on hematological parameters including hemoglobin[MCV], hematocrit [MCH], red and white blood cells count and corpuscle indices [MCHC], of cultured juvenile Beluga [Huso huso], with an initial body weight of 45 +/- 0.91g. After adaptation to a dry diet for 10 days group s, with 15 fish in each group, were used for this experiment. Each group was randomly distributed to twelve 500L fiberglass tanks. Each tank had a flow-through system. The fish were exposed to 4 different feeding regimes; Control Group [fed four times daily]; Group 1 [given 2 days of starvation]; Group 2 [given 4 days of starvation]; and, Group 3 [given 8 days of starvation]. Analysis was done at the end of each starvation period by collecting blood from the caudal vein and immediately transporting the samples to the laboratory. The results of the analysis indicated that the different starvation periods did not affect the red blood cell count. The hematocrit value in the Control Group was significantly lower [p<0.05] than the fish group starved for 8 days. MCHC value in the control group was significantly lower [p<0.05] than the fish group starved for 2 days. The hemoglobin concentration, MCV and MCH, did not show any significant difference between the treated groups and the Control Group. Those groups with longer starvation periods showed that the white blood cell count decreased significantly [p<0.05]. It could be concluded that short-term starvation periods weaken the immunological system of cultured juvenile Beluga